Pseudomonas aeruginosa culture [PSEUDO]
Published: March 21st, 2023
Revised: March 21st, 2023
Background
The Gram negative bacterium Pseudomonas aeruginosa is commonly found in soil, water, and other natural environments. It is an opportunistic pathogen that can cause infections in humans, particularly in individuals with weakened immune systems, such as those with cystic fibrosis or hospitalized patients. P. aeruginosa is known for its ability to form biofilms, which makes it difficult to treat with antibiotics. It is also resistant to many commonly used antibiotics, which can make infections difficult to treat. P. aeruginosa is a versatile organism that can thrive in a variety of environments and can cause a range of infections, from skin infections to pneumonia and sepsis.
Analysis
This test uses a semi-selective growth medium (Cetrimide Select agar) to detect and culture and differentiate P. aeruginosa from water samples. Results of this test are reported quantitatively as CFUs of P. aeruginosa per 100 mL.
Sample collection procedure
- Collect 500 mL of water in clean, new, screw-top bottle. For water sources expected to contain disinfectant chemicals such as chlorine, the collection container should include a suitable preservation buffer if biocides are likely to be present (the US-CDC recommends sodium thiosulfate to a final concentration of 0.1 M). We are pleased to provide sample collection containers to meet your needs. Please contact us prior to sampling to make arrangements.
- Transport samples to the laboratory as soon as possible after collection. Samples may be transported at room temperature but must be protected from temperature extremes. Samples not processed with 24 hours of collection must be refrigerated.
Laboratory code: PSEUDO
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